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Saturday, July 11, 2020 | History

2 edition of Observations on the freezing anf thawing of biological systems. found in the catalog.

Observations on the freezing anf thawing of biological systems.

George Kerr Rennie

Observations on the freezing anf thawing of biological systems.

by George Kerr Rennie

  • 327 Want to read
  • 8 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1969.

ID Numbers
Open LibraryOL13844872M

  Our observations indicate that recombinant adenoviral vector was more stable than expected both during freezing and thawing and during storage at low temperatures. Our results confirm the importance of appropriate conditions for the delivery and transport of recombinant adenoviral by: Freezing point depression and freeze-thaw damage by nano- and the function of biological systems. In most cases, the ions are assumed to have negligible solubility in the frozen solid, as is the case with to complete freezing11,16, In biological cells, as shown in Figure 2(d), electrolytes are con- Author: Tingtao Zhou, Mohammad Mirzadeh, Roland J.-M. Pellenq, Martin Z. Bazant.

Repeated freezing and thawing, however, This observation thus renders nick formation due to physical shearing by growing ice crystals the most likely damage type to contribute to the limited DNA origami stability during repeated freezing and thawing. which is one of the most widely used cryoprotectants for biological systems. Author: Yang Xin, Charlotte Kielar, Siqi Zhu, Christoph Sikeler, Xiaodan Xu, Christin Möser, Christin Möser. These problems are further divided into boundary conditions of temperature, prescribed heat flux, and surface convection. Chapter 5 examines some plane geometries involving three-dimensional freezing or thawing. Problems in the cylindrical and spherical coordinate systems are covered in chapters 6 and 7.

Biopolymer-based composition with adding of conductive polymer poly-(3,4-ethylenedioxythiophene) polystyrenesulfonate (PEDOT PSS) was made by mixing of iota-carrageenan (CRG), polyvinyl alcohol (PVA) and PEDOT PSS followed by freezing/thawing cycles. The method is environmentally friendly and based on the formation of polymer matrix upon of mixing CRG, PVA and PEDOT PSS and formation Author: Pavel M. Gotovtsev, Pavel M. Gotovtsev, Gulfiya U. Badranova, Yan V. Zubavichus, Nikolay K. Chumakov. One-Dimensional Freezing System by a Perturbation Method," ASME Trans., J. of Heat Transfer, pp. , May 4) B. Rubinsky and E.G. Cravalho, "Analysis for the temperature Distribution During the Thawing of a Frozen Biological Organ," E. Symposium "Cryomicroscopic Observation on Directional Solidification in Onion Cells.


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Observations on the freezing anf thawing of biological systems by George Kerr Rennie Download PDF EPUB FB2

Schematic diagrams of the experimental setup, cryoprobe and thermocouples placement to study the cryo-freezing of a biological system. For the first stage of the experiment, the freezing specimen was represented using a bio-gel. The bio-gel was prepared by dissolving SeaKem LE Cited by: Simplified calculation of cell water content during freezing and thawing in nonideal solutions of cryoprotective agents and its possible application to the study of.

ZETA Freeze & Thaw Systems are an economical and rational solution that offers a permanent improvement in the way we do scale-up and design of industrial freezing and thawing processes. The system approach covers the complete sequence from first laboratory experiments in the LabFreeze through controlled scale-up in the PilotFreeze and then to.

This book is divided into 23 chapters and begins with descriptions of several low temperature processes, such as nucleation, ice crystal growth, and freezing. The succeeding chapters deal with the protective mechanisms in frost-hardy plants, the physico-chemical changes in foods during freezing and storage, and the influence of cold storage, freezing, and thawing microbial and population of several foodstuffs.

Chapter 5 examines some plane geometries involving three-dimensional freezing or thawing. Problems in the cylindrical and spherical coordinate systems are covered in chapters 6 Format: Ebook.

This book serves as an introduction to the continuum mechanics and mathematical modeling of complex fluids in living systems.

The form and function of living systems are intimately tied to the nature of surrounding fluid environments, which commonly exhibit nonlinear and history dependent responses. This book is divided into 23 chapters and begins with descriptions of several low temperature processes, such as nucleation, ice crystal growth, and freezing.

The succeeding chapters deal with the protective mechanisms in frost-hardy plants, the physico-chemical changes in foods during freezing and storage, and the influence of cold storage, freezing, and thawing microbial and Book Edition: 1.

PDF | On Aug 2,J.-J. Chang and others published Freezing, sectioning and observation artefacts of frozen hydrated sections for electron microscopy | Find, read and cite all the research you. Figure 1, panel B and Fig. 2 panels D and F show the geometry and installation pattern of the heating array.

A total of heaters were emplaced in 11 Cited by: 2. This book on frozen food, as its title suggests, is written for the food technologist and food scientist in the frozen food industry, which includes both food and equipmentmanufacturers.

The information will also be useful for otherdisciplines within the food industry as awhole, and for studentsoffood technology.

The book, the aimofwhich is to provide an up-to-date reviewofthe technologyofthe Reviews: 1. A general observation in the cryopreservation of cells and other biological systems is that each system has a specific optimal cooling rate, with decreased survival at cooling rates that are too low (slow-cooling damage) or too high (fast-cooling damage) (Mazur et al., ).File Size: KB.

G.N. Ling and Z.L. Zhang, Studies on the physical state of water in living cells and model systems IV. Freezing and thawing point depression of water by gelatin, oxygen containing polymers and urea denatured proteins, Physiol.

Chem. Phys. Med. NMR (). PubMed Google ScholarCited by: 1. Chapter 5 examines some plane geometries involving three-dimensional freezing or thawing. Problems in the cylindrical and spherical coordinate systems are covered in chapters 6 and 7. Chapter 8 is an introduction to solidification in porous : V.

Lunardini. Cambridge Core - Obstetrics and Gynecology, Reproductive Medicine - Fertility Cryopreservation - edited by Ri-Cheng Chian. Full text of "Effects of freezing and frozen storage on histologic characteristics of canine tissues" See other formats EFFECTS OF FREEZING AND FROZEN STORAGE ON HISTOLOGIC CHARACTERISTICS OF CANINE TISSUES by MARTHA A.

BARAIBAR D.V.M. Universidad de la Republica Montevideo, Uruguay, A MASTER'S THESIS submitted in partial fulfillment of the. Mazur P. Cryobiology: the freezing of biological systems. Science. May 22; ()– Mazur P, Miller RH, Leibo SP.

Survival of frozen-thawed bovine red cells as a function of the permeation of glycerol and sucrose. J Membr Biol. ; 15 (2)– Mazur P, Schmidt by:   Secondly, the sample was cooled slowly to −20 °C before cooling it further to −80 °C, then slowly thawed at room temperature (condition B: slow freezing/slow thawing).

Thirdly, we combined rapid freezing (step 1 from condition A) with slow thawing (step 2 from condition B) such that fast freezing is followed by slow thawing (condition C).Cited by: The various studies carried out under high pressure freezing and thawing have been mentioned in Table 3.

and biological systems including human dermal fibroblast cells, porcine arteries and. after six freeze/thaw cycles. One thawing did not change the concentration of MMP-7 and VEGF-receptor.

However, repeated freeze/thaw cycles increased the measured values significantly. Decreases in VEGF concentrations were dramatic, even after the first freeze/thaw cycle. Clin Chem Lab Med. Feb;49(2) Epub Dec Size: KB.

The membrane rupture in plant tissue cells was higher after freezing and subsequent thawing (% of total area for potato cells and –% for sugar beet cells determined immediately after thawing), which increased substantially during the next 2 by:. This loss of viability is likely attributable to the two main biophysical mechanisms that occur during the freezing process in all biological systems, as described below.

All cell systems do share common cell freezing responses which may be exploited to better understand and alleviate the specific problems of by: 1.

Effect of thawing temperature and supplementation of 1 mg ml −1 9 kDa PVA in a mg ml −1 HES solution on cell recovery. mg ml −1 HES and 45 °C thaw Cited by:   One of the major concerns with a potential change in climate is that an increase in extreme events will occur.

Results of observational studies suggest that in many areas that have been analyzed, changes in total precipitation are amplified at the tails, and changes in some temperature extremes have been observed.

Model output has been analyzed that shows changes in extreme Cited by: